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SiRNA is designed to be perfectly complementary to the target mRNA and, miRNA follows the "seed pairing rule", a complementary binding of miRNA seed region to binding site (BS) located in the mRNA 3' untranslated region (3'UTR) (Figure 1C) The seed region involves nt 28 from miRNA 5' end or possibly nt 27 and 26The seed region comprises 6 nucleotides in positions 2–7 of the antisense siRNA strand of the siRNA duplex Matches such as these can contribute to downregulation of unintended targets due to the siRNA mimicking the action of an miRNA siRNA designed at QIAGEN is analyzed for 3' UTR/seed region complementarity using a proprietary set of 3' UTR sequences derived from theONTARGETplus siRNA utilizes groundbreaking peerreviewed research from Dharmacon, published in 06, which was the first to demonstrate that matches between an siRNA seed region (positions 27 of antisense strand) and an mRNA 3' untranslated region (UTR) are associated with offtarget si...